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. 2013 Jan 28;6:1–13. doi: 10.4137/CGM.S11124

Figure 6.

Figure 6

The lethality of the combination therapy of mifepristone and LY294002 is observed in cultures of ovarian cancer spheroids. In the upper panel (A), cells were plated in equivalent numbers in 8-well slides with Matrigel in media supplemented with growth factors. Media was changed every 4 days. Representative phase contrast images are shown. Scale bar = 20 μm. In the lower panels, cells were cultured for 72 hours in the presence of vehicle (VEH; B), 20 μM mifepristone (MF; C), 10 μM LY294002 (LY; D), or a combination of 20 μM MF+ 10 μM LY (E). Cells were incubated with calcein AM and EthD-1 without fixation. Live cells were defined by a bright green fluorescence and dead cells by a bright red fluorescence. (F) A positive control of spheroids containing dead cells was generated upon exposure for 1 hour to 20 μM cisplatin (CDDP). Scale bar = 50 μm.