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. 2012 Dec 4;12(12):16660–16672. doi: 10.3390/s121216660

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© 2012 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 2. — Mobility shift assay of QD655 conjugation with capturing probes. M: 1 kb DNA ladder, lane 1: QD655 particles only, lane 2: QD655 modified with sulfo-SMCC crosslinker, lane 3: sulfo-SMCC modified QD655 conjugated with capturing probes. The QD655 particles surrounded with positively charged amine-derivatized PEG do not migrate very quickly (lane 1). The binding of sulfo-SMCC on the QD655 increases the mobility of the conjugated particles since sulfo-SMCC possesses a negatively charged sulfonate group on its NHS ring (lane 2). The binding of capturing DNA probes to sulfo-SMCC modified QD655 particles further increases the mobility of the conjugates due to the additional negative charge introduced by the oligonucleotide probes (lane 3), verifying the conjugation of QD655 with capturing probes.