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. 2012 Dec 4;12(12):16685–16694. doi: 10.3390/s121216685

Figure 1.

Figure 1.

The detection of TMV in soil by the assays of DAS-ELISA (A), RT-PCR (A) and IC-real-time RT-PCR (B). Lanes 1 to 6 are the detections of TMV in one gram soil samples artificially blended with different dilutions naturally TMV-infected leaf at 1:101, 1:102, 1:103, 1:104, 1:105 and 1:106 (1 mL, w/v, g/mL), respectively, and, Lanes 7 to 9 are the detection of TMV from ToMV-contaminated soil, TMV-free soil, and healthy leaf extract, respectively. “+” represents positive result of DAS-ELISA for detecting TMV, “−” represents negative result of DAS-ELISA for detecting TMV. (B) Sensitivity of the IC-real-time RT-PCR assay as monitored by amplified curve. Shown from left to right are the curves of soil contamined with decreasing TMV-infected leaf saps at 1:101, 1:102, 1:103, 1:104, 1:105 and 1:106 (1 mL, w/v, per g soil) dilutions. The curves roughly parallel to the X axis are TMV from ToMV-contaminated soil, TMV-free soil, and healthy leaf extract, respectively.