Table 1.
Plasmodium falciparum false-negative diagnoses by the HRP2-based test
| Palutop+4® | Microscopy | RT-PCR | HRP2a | HRP3b | |||||
|---|---|---|---|---|---|---|---|---|---|
| Identification |
Pan |
Pf |
Pv |
Diagnostic |
Parasitaemia |
Pf |
Pv |
|
|
| 186/1 |
+ |
− |
+ |
Pf |
0.5% |
+ |
− |
− |
− |
| 212/1 |
− |
− |
− |
Pf |
0.02% |
+ |
− |
+ (B) |
− |
| 55/2 |
− |
− |
− |
Pf |
0.5% |
+ |
− |
+ (B) |
− |
| 56/3 |
− |
− |
− |
Pf |
0.5% |
+ |
− |
− |
+ |
| 150/3 |
− |
− |
− |
Pf |
0.001% |
+ |
− |
− |
− |
| 157/3 |
− |
− |
− |
−c |
|
+ |
− |
+ (B) |
− |
| 183/3 | − | − | − | Pf | 0.004% | + | − | + (C) | − |
Pf = Plasmodium falciparum; Pv = Plasmodium vivax; RT-PCR = real-time polymerase chain reaction.
a − If deletion of pfhrp2 and + if presence of pfhrp2.
(B) Group B, sensitive to PfHRP2, detection, number of type 2 repeats × type 7 repeats between 50 to 100 repeats.
(C) Group C, non sensitive PfHRP2 detection, number of type 2 repeats × type 7 repeats < 43 repeats.
b − If deletion of pfhrp3 and + if presence of pfhrp3.
c Thin and thick blood smears were negative.