Table 1.

Comparison of assay parameters from bioluminescence and fluorescence-based assays

Assay reagent	Z’score	Signal/noise ratio	%CVmax	%CVmin
Bright-Glo	0.74–0.84	15667–17746	5.2–6.7	14.8–16.2
SL substrate1	0.73–0.81	12853–13609	8.0–8.9	15.5–17.0
One-Glo	0.77–0.86	8936–10030	4.7–6.3	14.3–19.5
Steady-Glo	0.83–0.86	2063–2344	5.6–7.1	17.2–18.6
MSF	0.72–0.79	4.30–4.39	4.0–6.6	2.8–5.8

¹Standard luciferase (SL).