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. 2012 Dec 17;8:49. doi: 10.1186/1746-4811-8-49

Figure 5.

Figure 5

A generic workflow of batch processing flg22-induced callose deposits image sequence using CalloseMeasurer. (A) A series of callose images are batch processed by CalloseMeasurer (v1.0). (B) CalloseMeasurer detects callose deposits (highlighted in green) and identifies spreading callose networks (optional, coloured red). Generated images are saved as PNG files. Quantifiable callose features such as size, shape, fluorescence signal intensity, and area/length of spreading callose networks are exported to two CSV files for further statistical analysis.