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. 2013 Feb 1;10:13. doi: 10.1186/1742-4690-10-13

Figure 2.

Figure 2

IN interacts with cellular cofactors LEDGF/p75 and TNPO3 at 6 h p.i. SupT1 cells were not infected (NI) or infected (I) with HIV-1IN-HA. WCE were prepared at 2 h and 6 h p.i. (as indicated), immunoprecipitated (IP-HA) with the anti-HA affinity matrix and analyzed by Western blotting using antibodies against HA, LEDGF/p75, and TNPO3. Inputs represent 5& of the immunoprecipitated material. To detect IN-HA at 2h p.i. the film was exposed for 5 minutes whereas detection of IN-HA at 6h p.i. required film exposure of 30 minutes. Dividing lines indicate the grouping of parts of the same gel.