Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Feb 1;10:13. doi: 10.1186/1742-4690-10-13

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright ©2013 Gérard et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

IN complex II and III are exclusively nuclear. Control SupT1 cells TC3 (A) and LEDGF/p75-knock-down SupT1 cells (TL34) (B) were infected with HIV-1_IN-HA. Cytosolic and nuclear extracts (CE and NE, respectively) were prepared at 2 h p.i. and subjected to gel filtration on a Superdex 200HR 10/300 column. Fractions were collected and analyzed by Western blotting using antibodies against LEDGF/p75, or HA. Inputs represent 3,5& of WCE load. LEDGF/p75 served as an internal control for the nuclear fraction in (A).