Figure 2. Immunostaining of βIII-tubulin and actin in growth cones from control and axotomized sensory neurons.

(A) Confocal images of double immunostaining with anti- βIII-tubulin (Tub, green) and anti-actin antibodies (Actin, red) in control sensory neuron at 1DIV. Merged image shows preferential actin localization to growth cone tip. (B) Confocal images of double immunostaining with anti- βIII-tubulin and anti-actin antibody in axotomized sensory neuron at 1DIV. Neurite and growth cone are larger than in control and merged image shows a slight actin spreading as observed in control in combination with a very thin tubulin prolongation at the growth cone tip. (Scale bars 5 µm, ×63).