Figure 3. The effect of NDRG1 on apoptosis. (A) Subconfluent cells were challenged with indicated reagents for 24 h. Total cell lysate (including the floaters) was subjected to western blot analysis to detect the intact (p116) and cleaved (p85) forms of PARP. (B) Caspase3/7 activities were determined after 24 h treatment of indicated reagents, and were plotted as fold induction against untreated cells. Abbreviations for treatments: Doxo: 2 μM doxorubicin; Cis: 5 μg/mL cisplatin; Ni: 1 mM NiSO₄; Co: 1 mM CoCl₂. Columns, mean of the normalized data from three independent experiments; Bars, ± SD; * p < 0.05 vs. wild type control.