Figure 1. Romidepsin induced CXCR4 mRNA overexpression in human cancer cell lines. (A) CXCR4 expression levels were measured by semiquantitative RT-PCR in renal cancer cell lines UOK121, UOK143 and UOK181 treated with romidepsin (10 ng/ml) + verapamil (5 μg/mL) for 24 h, with or without decitabine (1 mM) daily for four days. (B) CXCR4 mRNA expression in SF295, H460, SW620 and MCF7cells treated with romidepsin (20 ng/ml for 24 h) + verapamil (5 μg/mL). GAPDH was used as the internal control. Numbers indicate fold increase of CXCR4 relative to the untreated cells. Representative results from three independent experiments are shown.