Figure 2. Multi-Response Permutation Procedure (MRPP) and Canonical Correspondence Analysis (CCA) performed on eukaryotic rRNA OTUs sharing 80–97% sequence identity, as well as fungal TRFLP patterns (denoted as ‘TRFLP’ on the right hand side of each plot).

(A) MRPP analysis of the combined effect of TOC, DIC, and sulfide and (B) sediment depth on subsurface eukaryotic distributions. Statistically significant p-values (<0.05) are highlighted. CCA correlation values (R²) of TOC (C), sulfide (D), and DIC (E) with different eukaryotic taxa in subsurface samples. (F) OTU richness of eukaryotic taxa detected in marine subsurface samples.