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. 2013 Feb 13;8(2):e56093. doi: 10.1371/journal.pone.0056093

Figure 2. 10-plex PCR profiles generated from representative Ba and BaNN strains.

Figure 2

As input to PCR, 100 copies of template Ba strains Sterne, Ames, and BA1035; and 1000 copies of template BaNN strains Bc E33L and Bt 97-27, were used. The FAM (blue), JOE (green), TMR (black) and ROX (red) labeled products are aligned to illustrate amplicon sizes from the set of five strains. As expected, all 10 loci were amplified in the 3 Ba strains; only 6 of 10 loci were observed in BaNNs. The basB and pbp1A amplicons allow discrimination between strains Ames, Sterne, and BA1035; pbp1A fragment length also distinguishes Bc E33L from Bt 97-27. X-axes show fragment size in base pairs and Y-axes relative fluorescence units (rfu).