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. 2013 Feb 13;8(2):e56093. doi: 10.1371/journal.pone.0056093

Figure 3. 10-plex PCR profiles generated from representative Ft strains (left panel) and Yp and YpNN strains (right panel).

Figure 3

The FAM (blue), TMR (black) and ROX (red) labeled products are aligned to emphasize the variant and non-variant fragment sizes observed across these strains. Six of ten fragments (acpA, pepO, migR, fopA, tul4-FTT0900, and iglC) in the 10-plex Ft panel are invariable in length between strains subsp. tularensis SchuS4 and WY96, and subsp. holarctica 14LVS and 425. In Yp 10-plex panel, size variability is observed primarily in 4 loci (glpD, ail, argS-YPO2047 and YPO1976). Both Ft 10-plex and Yp 10-plex panels do not have any JOE-labeled (green) primers. For each reaction, 100 genome equivalents of each strain were used as template to PCR.