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. 2013 Feb 13;8(2):e56093. doi: 10.1371/journal.pone.0056093

Figure 4. 30-plex PCR profiles generated from select biothreat agents Ba, Ft, and Yp.

Figure 4

Electropherogram obtained from 100 copies of Ba strain Sterne (A); from 1000 copies of Ft subsp. tularensis WY96 (B); and from 100 copies of Yp bv. mediavalis strain KIM10v (C). Note that 1000 copies of Ft DNA are required to detect two of the FAM-labeled targets (acpA and speA) due to their high AT content resulting in reduced PCR efficiency.