Table 2. hAFLP types and Sequence Types (STs) of Ft strains and Ft near neighbor species DNA (shown in bold) and by in silico analysis of WGS (shown in italics).
Strain [Genbank Acc#] | hAFLP | ST | iglC | fopA | tul4-FT900 | pdpD | acpA | pepO | migR | gyr | speA | FT82 | ||||||||||
subspecies tularensis | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | ||
Schu S4 [AJ749949.2] | a | A | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
FSC198 [AM286280.1] | a | A | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
FSC033 [AAYE00000] | a | A | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
MA00-2987 [ABRI00000] | a | A | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
NE061598 [CP001633] | c | C | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
Scherm | c | C | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
tularensis FRAN005 | c | C | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
tularensis FRAN006 | c | C | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
tularensis FRAN007 | c | C | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
tularensis FRAN008 | c | C | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
tularensis FRAN009 | c | C | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
tularensis FRAN011 | c | C | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
tularensis FRAN015 | c | C | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
WY96 [CP000608.1] | b | B | 1 | 2 | 1 | 3 | 1 | 2 | 1 | 2 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 |
ATCC 6223 | B | B | 1 | 2 | 1 | 3 | 1 | 2 | 1 | 2 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 |
subspecies holarctica | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | ||
LVS [AM233362.1] | d | D | 1 | 2 | 1 | 2 | 1 | 3 | n | n | 1 | 2 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 3 | 3 | 3 |
14LVS | d | D | 1 | 2 | 1 | 2 | 1 | 3 | n | n | 1 | 2 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 3 | 3 | 3 |
LVSG | d | D | 1 | 2 | 1 | 2 | 1 | 3 | n | n | 1 | 2 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 3 | 3 | 3 |
JAP | d | L | 1 | 2 | 1 | 2 | 1 | 3 | n | n | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 4 | 3 | 3 |
FSC257 [AAUD00000] | d | F | 1 | 3 | 1 | 2 | 3 | 4 | Δ | Δ | 1 | 2 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 3 | 3 | 3 |
VT68 | E | K | 1 | 2 | 1 | 2 | 1 | 3 | n | n | 1 | 2 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 3 | 3 | 3 |
holarctica FRAN012 | e | K | 1 | 2 | 1 | 2 | 1 | 3 | n | n | 1 | 2 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 3 | 3 | 3 |
KY99-3387 | e | K | 1 | 2 | 1 | 2 | 1 | 3 | n | n | 1 | 2 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 3 | 3 | 3 |
OSU18 [CP000437.1] | e | E | 1 | 2 | 1 | 5 | 1 | 3 | Δ | Δ | 1 | 2 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 3 | 3 | 3 |
FSC200 [AASP00000] | e | J | 1 | 2 | 1 | 2 | 1 | 3 | Δ | Δ | 1 | 2 | 1 | 1 | 1 | 2 | 2 | 6 | 2 | 3 | 3 | 3 |
FSC022 [AAYD00000] | f | G | 1 | 2 | 1 | 2 | 1 | 3 | Δ | Δ | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 5 | 5 | 3 | 3 |
URFT1 [ABAZ00000] | g | H | 1 | 2 | 1 | 2 | 1 | 3 | Δ | Δ | 1 | 2 | 2 | 4 | 1 | 2 | 1 | 1 | 3 | 6 | 3 | 3 |
FTNF002-00 [CP00083.1] | h | I | 1 | 2 | 1 | 2 | 1 | 3 | Δ | Δ | 1 | 2 | 2 | 4 | 1 | 2 | 1 | 1 | 2 | 3 | 3 | 3 |
425 | n | M | 1 | 2 | 1 | 4 | 1 | 3 | n | n | 1 | 2 | 1 | 1 | 1 | 2 | 4 | 4 | 2 | 3 | 4 | 4 |
OR96-03246 | o | R | 1 | 2 | 1 | 4 | 1 | 3 | n | n | 1 | 2 | 1 | 1 | 1 | 2 | 3 | 3 | 2 | 3 | 4 | 4 |
subspecies mediasiatica | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | ||
FSC147 [CP000915.1] | i | N | 1 | 4 | 1 | 6 | 3 | 4 | 1 | 3 | 1 | 1 | 3 | 3 | 1 | 1 | 5 | 5 | 4 | 7 | 3 | 5 |
subspecies novicida | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | ||
KM14S | k | O | 1 | 2 | 1 | 2 | 2 | 5 | 1 | 4 | 1 | 3 | 1 | 6 | 1 | 3 | n | n | n | n | 4 | 6 |
GB2 | k | O | 1 | 2 | 1 | 2 | 2 | 5 | 1 | 4 | 1 | 3 | 1 | 6 | 1 | 3 | n | n | n | n | 4 | 6 |
CG21 | k | O | 1 | 2 | 1 | 2 | 2 | 5 | 1 | 4 | 1 | 3 | 1 | 6 | 1 | 3 | n | n | n | n | 4 | 6 |
U112 [CP000439.1] | k | O | 1 | 2 | 1 | 2 | 2 | 5 | 1 | 4 | 1 | 3 | 1 | 6 | 1 | 3 | Δ | Δ | Δ | Δ | 4 | 6 |
FTE [ABSS00000] | k | O | 1 | 2 | 1 | 2 | 2 | 5 | 1 | 4 | 1 | 3 | 1 | 6 | 1 | 3 | Δ | Δ | Δ | Δ | 4 | 6 |
FTG [ABXZ00000] | k | U | 1 | 2 | 1 | 2 | 2 | 5 | 1 | 4 | 1 | 3 | 1 | 5 | 1 | 4 | Δ | Δ | Δ | Δ | 4 | 6 |
Fx1 [CP002557] | j | V | 1 | 5 | 1 | 8 | 1 | 8 | 1 | 4 | 1 | 4 | 1 | 1 | 1 | 5 | Δ | Δ | 2 | 10 | 4 | 8 |
3523 [CP002558] | m | S | 1 | 8 | 1 | 9 | 1 | 9 | 1 | 5 | 1 | 5 | 1 | 7 | 1 | 6 | Δ | Δ | Δ | Δ | 4 | 7 |
GA99-3548 [ABAH00000] | l | P | 1 | 6 | 1 | 8 | 1 | 7 | 1 | 6 | 1 | 4 | 1 | 5 | 1 | 4 | 3 | 7 | 2 | 8 | n | n |
GA99-3549 [AAYF00000] | p | Q | 2 | 7 | 1 | 7 | 1 | 6 | n | n | 1 | 4 | 1 | 6 | 1 | 4 | 1 | 8 | 2 | 9 | 4 | 6 |
F. philomiragia | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | FLT | GT | ||
ATCC 25015 [ABYY00000] | – | – | n | n | n | n | n | n | 1 | 8 | n | n | n | n | n | n | n | n | n | n | n | n |
ATCC 25016 | – | – | n | n | n | n | n | n | 1 | 9 | n | n | n | n | n | n | n | n | n | n | n | n |
ATCC 25017 [CP000937.1] | – | – | n | n | n | n | n | n | 1 | 9 | n | n | n | n | n | n | n | n | n | n | n | n |
Corresponding Genbank accession numbers are in brackets. FLT: Fragment Length Type; GT: sequence genotype of individual locus; Δ: strain is deficient of this locus; n: no PCR product or sequence detected.