Table 1. Frequency of coffee phenotypic variants detected in the nursery and field on three year-old plants from two C. arabica hybrids depending on the type of proliferation system used in the industrial somatic embryogenesis process.

Proliferation system in the somatic embryogenesis process	Hybrid	Observations after 10 months in nursery				Observations after 36 months in field
		No. of observed emblings	No. of variants	Somaclonal variation frequency (%)	3σ confidence interval*	No. of emblings	No. of variants	Somaclonal variation frequency (%)	3σ confidence interval*
Secondary embryogenesis	H1	117.115	148	0.13	[0.09–0.16]	51.131	373	0.73	[0.62–0.84]
	H3	121.894	159	0.13	[0.10–0.16]	49.126	390	0.79	[0.67–0.91]
	Total	239.009	307	0.13	[0.10–0.15]	100.257	763	0.76	[0.67–0.84]
Embryogenic suspension	H1	204.871	206	0.10	[0.08–0.12]	54.218	402	0.74	[0.63–0.85]
	H3	197.705	183	0.09	[0.07–0.11]	54.566	394	0.72	[0.61–0.83]
	Total	402.576	389	0.09	[0.08–0.11]	108.784	796	0.73	[0.65–0.80]

The variable analyzed was the proportion (p) of variant (p = X/n), where X was the number of variant and n the number of plants observed. A 3σ confidence limit for binomial distribution was calculated using the formula with a level of confidence of 99%.