Table 6. Overall MSAP data and methylation polymorphism among mother plants and emblings of C. arabica hybrids derived from secondary embryogenesis or embryogenic suspensions.

Proliferation in the somatic embryogenesis process	Hybrid	No. of emblings analyzed	No. of fragments	Methylation polymorphic fragments		Total polymorphism*(%)	3σ confidence intervals**
				No.	Percentage (%)
Secondary embryogenesis	H1	33	399	5	1.2	0.18	[0.071–0.294]
	H3	45	396	7	1.7	0.16	[0.068–0.246]
		78		12	1.5	0.17	[0.098–0.238]
Embryogenic suspensions	H1	26	399	4	1.0	0.18	[0.057–0.309]
	H3	41	396	5	1.0	0.07	[0.006–0.129]
		67		9	1.1	0.11	[0.051–0.174]

^*Total polymorphism  =  [No. of methylation polymorphic fragments/(No. of fragments × No. emblings)]×100.

^**The variable analyzed was the proportion (p) of methylation polymorphisms (p = X/n), where X was the number of methylation polymorphisms and n the total number of fragments. A 3σ confidence limit for binomial distribution was calculated using the formula with a level of confidence of 99%.