Figure 3. RT-PCR amplification of mRNA produced by C. cellulolyticum wild type or overproducing constitutive regulator.

mRNA were prepared from cultures of H10, H10(pSOSzero), and H10(pSOS954Δ116) grown on cellulose or straw as indicated and reverse transcribed. PCR was performed on the cDNA with the following pairs of primers (Table S1): RPO-F/RPO-R (Table S1) targeting a housekeeping gene used for standardization and quantification of the induction (RPO), 1229rtD/1229rtR (Table S1) targeting end of the first gene and beginning of the second gene of xyl-doc cluster (29–30), 1230rtD/1231rtR (Table S1) targeting end of the second gene and beginning of the third gene of xyl-doc cluster (30–31), and 1656rtD/1656rtR (Table S1) targeting upstream sequence and beginning of the gene at locus Ccel_1656 (1656).