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. 2013 Feb 13;8(2):e56063. doi: 10.1371/journal.pone.0056063

Figure 4. qRT-PCR analysis of mRNA produced by xydR knock-out strain.

Figure 4

mRNA were prepared from cultures in straw-based medium of wild-type (H10), MTL1228(pSOSzeroTc) and MTL1228(pSOS955Δ116) strains and reverse transcribed. qPCR was performed on the cDNA with the following pairs of primers :1229qRT-F/1229qRT-R (Table S1) targeting the first gene of xyl-doc cluster at locus Ccel_1229, 1656qRT-F/1656qRT-R (Table S1) targeting the gene at locus Ccel_1656. Levels of expression of genes at loci Ccel_1229 and Ccel_1656 are given after standardization with the level of expression of rpoD (using the primers RPO-F and RPO-R, Table S1). Error bars indicate the standard deviation of three independent qPCR reactions. Dark grey represents the results obtained with mRNA from H10 strain, light grey are the ones with mRNA from MTL1228(pSOSzeroTc) strain and grey ones with mRNA from MTL1228(pSOSΔ116) strain.