. 2013 Feb 13;8(2):e56063. doi: 10.1371/journal.pone.0056063

Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid	Relevant feature(s)	Source or reference
E. coli BL21(DE3)	F⁻ ompT dcm hsdS_B(r_B ⁻ m_B ⁻) gal lon λDE3	Novagen
E. coli DH5α	F⁻Φ80 lacZΔM15Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(r_K ⁻ m_K ⁺) phoA supE44 thi-1 gyrA96 relA1 λ⁻	Invitrogen
E. coli JM109	recA1 mcrB⁺ hsdR17	Promega
C. cellulolyticum H10	wild-type strain, ATCC35319/DSM 5812	DSMZ
C. cellulolyticum MTL1228	ATCC35319 derivative, xydR ::intron, Em^r	This study
pAM120	tetM from E. faecalis carrying vector; Tc^r	[46]
pET22b(+)	E. coli expression vector (T7 promoter); Ap^r	Novagen
pET1230GH10	pET22b(+) derivative carrying 0.9-kb NdeI-XhoI fragment of locus Ccel_1230 encoding GH10 domain	This study
pET1234	pET22b(+) derivative carrying the 1.5-kb NcoI-XhoI fragment of locus Ccel_1234	This study
pET1237	pET22b(+) derivative carrying the 1.7-kb NdeI-XhoI fragment of locus Ccel_1237	This study
pET1656Unk	pET22b(+) derivative carrying the 2.2-kb NdeI-XhoI fragment of locus Ccel_1656 encoding unknown domain	This study
pGEMT-Easy	E. coli cloning vector; Ap^r	Invitrogen
pJIR418	catP from C. perfringens carrying vector, Tm^r	[47]
pMAL-c2x	E. coli MBP fusion vector (tac promoter, malE); Ap^r	New England Biolabs
pMALΔ116	pMAL-c2x derivative carrying 1.2-kb BamH1-Pst1 fragment containing deleted xydR	This study
pMTL007	E. coli/Clostridium shuttle vector (ColE1, pCB102) Ll.ltrBintron (ermBtdRAM2) under the controlof P_fac, ltrA; Cm^r/Tm^r	[21]
pMTL007xydR	pMTL007 derivative targeting xydR (locus Ccel_1228)	This study
pSOS954	E. coli/Clostridium shuttle expression vector, (ColE1, pIM13), P_thl; expression cassette fromC. acetobutylicum, Ap^r, Em^r	[13]
pSOSzero	E. coli/Clostridium shuttle vector (ColE1, pIM13); Ap^r, Em^r	[13]
pSOSzeroTm	pSOSzero derivative; Ap^r, Tm^r	This study
pSOSzeroTc	pSOSzeroTc derivative; Ap^r, Tc^r	This study
pSOS954Δ116	pSOS954 derivative carrying 1.3-kb BamH1-NarI fragment containing P_thl-xydR; Ap^r, Em^r	This study
pSOS955	pSOS954 derivative, P_thl; expression cassette from C. cellulolyticum; Ap^r, Tc^r	Generous gift from S. Perret
pSOS955Δ116	p955 derivative carrying 1.3-kb BamH1-NarI fragment containing P_thl-xydR; Ap^r, Tc^r	This study

Ap^r, ampicilline resistance; Tc^r, tetracycline resistance, Cm/Tm^r, chloramphenicol/thiamphenicol resistance; Em^r, erythromycine resistance.