Table 1. Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant feature(s) | Source or reference |
| E. coli BL21(DE3) | F− ompT dcm hsdSB(rB − mB −) gal lon λDE3 | Novagen |
| E. coli DH5α | F−Φ80 lacZΔM15Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rK − mK +) phoA supE44 thi-1 gyrA96 relA1 λ− | Invitrogen |
| E. coli JM109 | recA1 mcrB+ hsdR17 | Promega |
| C. cellulolyticum H10 | wild-type strain, ATCC35319/DSM 5812 | DSMZ |
| C. cellulolyticum MTL1228 | ATCC35319 derivative, xydR ::intron, Emr | This study |
| pAM120 | tetM from E. faecalis carrying vector; Tcr | [46] |
| pET22b(+) | E. coli expression vector (T7 promoter); Apr | Novagen |
| pET1230GH10 | pET22b(+) derivative carrying 0.9-kb NdeI-XhoI fragment of locus Ccel_1230 encoding GH10 domain | This study |
| pET1234 | pET22b(+) derivative carrying the 1.5-kb NcoI-XhoI fragment of locus Ccel_1234 | This study |
| pET1237 | pET22b(+) derivative carrying the 1.7-kb NdeI-XhoI fragment of locus Ccel_1237 | This study |
| pET1656Unk | pET22b(+) derivative carrying the 2.2-kb NdeI-XhoI fragment of locus Ccel_1656 encoding unknown domain | This study |
| pGEMT-Easy | E. coli cloning vector; Apr | Invitrogen |
| pJIR418 | catP from C. perfringens carrying vector, Tmr | [47] |
| pMAL-c2x | E. coli MBP fusion vector (tac promoter, malE); Apr | New England Biolabs |
| pMALΔ116 | pMAL-c2x derivative carrying 1.2-kb BamH1-Pst1 fragment containing deleted xydR | This study |
| pMTL007 | E. coli/Clostridium shuttle vector (ColE1, pCB102) Ll.ltrBintron (ermBtdRAM2) under the controlof Pfac, ltrA; Cmr/Tmr | [21] |
| pMTL007xydR | pMTL007 derivative targeting xydR (locus Ccel_1228) | This study |
| pSOS954 | E. coli/Clostridium shuttle expression vector, (ColE1, pIM13), Pthl; expression cassette fromC. acetobutylicum, Apr, Emr | [13] |
| pSOSzero | E. coli/Clostridium shuttle vector (ColE1, pIM13); Apr, Emr | [13] |
| pSOSzeroTm | pSOSzero derivative; Apr, Tmr | This study |
| pSOSzeroTc | pSOSzeroTc derivative; Apr, Tcr | This study |
| pSOS954Δ116 | pSOS954 derivative carrying 1.3-kb BamH1-NarI fragment containing Pthl-xydR; Apr, Emr | This study |
| pSOS955 | pSOS954 derivative, Pthl; expression cassette from C. cellulolyticum; Apr, Tcr | Generous gift from S. Perret |
| pSOS955Δ116 | p955 derivative carrying 1.3-kb BamH1-NarI fragment containing Pthl-xydR; Apr, Tcr | This study |
Apr, ampicilline resistance; Tcr, tetracycline resistance, Cm/Tmr, chloramphenicol/thiamphenicol resistance; Emr, erythromycine resistance.