Table 2. Detection of the products of xyl-doc and CBM6-containing proteins by LC-MS/MS in cellulosomesa.
Locus tag | Predicted functionb | Massc (kDa) | Quantitative valued | |||
straw | cellulose | cellulose | cellulose | |||
H10 | H10 (pSOSzero) | H10 (pSOSΔ116) clone 1 | H10 (pSOSΔ116) clone 2 | |||
Ccel_0728 | CipC Scaffolding protein | 156 | 831 | 1952 | 2131 | 2163 |
Ccel_1229 | xylanase/arabinosidase | 58 | 63 | 15 | 64 | 126 |
Ccel_1230 | xylanase | 60 | 90 | 35 | 152 | 158 |
Ccel_1231 | xylanase | 57 | 18 | 2 | 1 | 1 |
Ccel_1232 | feruloyl esterase | 53 | 58 | ni | 1 | 0 |
Ccel_1233 | xylosidase/arabinofuranosidase | 82 | 42 | 14 | 98 | 119 |
Ccel_1234 | α-arabinofuranosidase | 59 | 80 | 47 | 103 | 162 |
Ccel_1235 | xylanase | 56 | 46 | ni | 4 | 8 |
Ccel_1236 | unknown | 105 | 8 | 4 | 23 | 51 |
Ccel_1237 | Gal27A α-galactosidase | 65 | 20 | 9 | 52 | 70 |
Ccel_1238 | Gal59A α-galactosidase | 122 | 10 | ni | 16 | 20 |
Ccel_1239 | β-galactosidase | 110 | 18 | 12 | 55 | 37 |
Ccel_1240 | arabinofuranosidase/acetyl-xylan-esterase | 85 | 89 | 48 | 150 | 192 |
Ccel_1241 | α-fucosidase | 127 | 8 | 2 | 47 | 12 |
Ccel_1242 | xylanase/glucuronoxylanase | 68 | 30 | 15 | 80 | 61 |
Ccel_1656 | unknown | 103 | 35 | 2 | 134 | 119 |
Cellulosomes were produced by H10, H10(pSOSzero), H10(pSOS954Δ116) grown in cellulose- or straw-based media.
As given in Blouzard et al. 2010 [11].
Theoretical masses.
Quantitative values given as the spectral counts found by LC MS/MS analysis for identified proteins. ni, not identified.