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. 2013 Feb 13;8(2):e56063. doi: 10.1371/journal.pone.0056063

Table 2. Detection of the products of xyl-doc and CBM6-containing proteins by LC-MS/MS in cellulosomesa.

Locus tag Predicted functionb Massc (kDa) Quantitative valued
straw cellulose cellulose cellulose
H10 H10 (pSOSzero) H10 (pSOSΔ116) clone 1 H10 (pSOSΔ116) clone 2
Ccel_0728 CipC Scaffolding protein 156 831 1952 2131 2163
Ccel_1229 xylanase/arabinosidase 58 63 15 64 126
Ccel_1230 xylanase 60 90 35 152 158
Ccel_1231 xylanase 57 18 2 1 1
Ccel_1232 feruloyl esterase 53 58 ni 1 0
Ccel_1233 xylosidase/arabinofuranosidase 82 42 14 98 119
Ccel_1234 α-arabinofuranosidase 59 80 47 103 162
Ccel_1235 xylanase 56 46 ni 4 8
Ccel_1236 unknown 105 8 4 23 51
Ccel_1237 Gal27A α-galactosidase 65 20 9 52 70
Ccel_1238 Gal59A α-galactosidase 122 10 ni 16 20
Ccel_1239 β-galactosidase 110 18 12 55 37
Ccel_1240 arabinofuranosidase/acetyl-xylan-esterase 85 89 48 150 192
Ccel_1241 α-fucosidase 127 8 2 47 12
Ccel_1242 xylanase/glucuronoxylanase 68 30 15 80 61
Ccel_1656 unknown 103 35 2 134 119
a

Cellulosomes were produced by H10, H10(pSOSzero), H10(pSOS954Δ116) grown in cellulose- or straw-based media.

b

As given in Blouzard et al. 2010 [11].

c

Theoretical masses.

d

Quantitative values given as the spectral counts found by LC MS/MS analysis for identified proteins. ni, not identified.