Figure 1. Expression analysis of CaZF in response to various stresses in chickpea.

(A) Expression patterns of CaZF gene induced under different treatments. Total RNA was isolated from 10-d-old chickpea seedling treated with distilled water (control, Con), 100 µM abscisic acid (ABA), 4°C (Cold), dehydration (DH), 50 µM methyl-jasmonate (MeJA), 5 mM salicylic acid (SA), 250 mM NaCl (Salt) and wound for 5 h. (B) Time-course of accumulation of CaZF mRNA under dehydration, cold and salt treatments for 0.5 h, 1 h and 3 h. (C) Organ-specific expression of CaZF in root (R), shoot (S) and leaf (L) after cold, salt and dehydration treatments for 3 h. 20 µg of total RNA was electrophoresed on formaldehyde denaturing gel, blotted onto nylon membrane and hybridized with ³²P-radiolabeled CaZF cDNA. The lower panel in each figure shows ethidium bromide-stained ribosomal RNAs as loading controls. The relative intensities of the bands were quantitated by densitometry in PhosphorImager scanner and are presented in the form of fold changes below each blot.