Figure 5. Increased RhoA activity in hypoxic, P. aeruginosa infected cells is partly responsible for decreased internalization of P. aeruginosa in hypoxia.

A: Active RhoA (RhoA-GTP) was determined by ELISA in A549 cells pre-incubated in normoxia and hypoxia followed by infection with P. aeruginosa. Data represent mean ± SEM of 3 individual experiments (* p<0.05). B: Normoxic A549 cells were pre-treated with 10 µM Y27632 and throughout the following infection with P. aeruginosa. Internalized P. aeruginosa as % of normoxic control are shown. Data represent mean ± SEM of 5 individual experiments. C: Intracellular P. aeruginosa in A549 cells were determined in hypoxia with the same treatments as described in B. Internalized P. aeruginosa as % of normoxic control are shown. Data represent mean ± SEM of 5 individual experiments (* p<0.05).