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. 2013 Feb 13;8(2):e56465. doi: 10.1371/journal.pone.0056465

Figure 3. Complementation of the temperature sensitive mutant.

Figure 3

A GlyYP plate with 1, M2915-7C mgm101-1ts, and M2915-7C transformed with pCXJ22 plasmids containing, 2, S.cerevisiae MGM101, 3, A.millepora MGM101 with a S.cerevisiae mitochondrial targeting signal (A.m.ID-A.m.C)(Fig. 3), 4, S.cerevisiae intrinsically disordered (ID) domain joined to A.millepora core region (S.c.ID-A.m.C) and 5, A.millepora ID region joined to S.cerevisiae core region (A.m.ID-S.c.C). The constructs all have a mitochondrial targeting signal sequence as shown in Figure S2. The plate was incubated at 35°C for 3 days before being photographed.