Table 1. Distribution of phenotypes in segregants containing pCXJ22 MGM101 plasmids.
Plasmid Construct | Asci with 4 viable spores | Ratio of Gly+: Gly− spores* | Ura+ spores, frequency(%) | Ura+, Leu+ spores | |||
4∶0 | 3∶1 | 2∶2 | Gly+ | Gly− | |||
S.c MGM101 | 56 | 19 | 24 | 13 | 139 (62%) | 63 | 0 |
A.m MGM101 | 34 | 0 | 0 | 34 | 74 (54%) | 0 | 31 |
S.c 1D domain-A.m core* | 44 | 2 | 6 | 35 | 98 (56%) | 10 | 32 |
A.m 1D domain-S.c core | 50 | 0 | 0 | 50 | 108 (54%) | 0 | 44 |
1 tetrad contains 4 Gly− spores.