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. 2013 Feb 13;8(2):e56496. doi: 10.1371/journal.pone.0056496

Figure 2. Hedgehog stimulation suppresses apoptosis through the activation of Msi1.

Figure 2

hUCB-MSCs were cultured in serum-free medium and then treated with or without purmorphamine (4 µM). Apoptotic levels were assessed with a TUNEL assay and western blotting. Apoptotic cells were quantified by counting the number of TUNEL-positive cells. Purmorphamine treatment for 72 h significantly decreased the number of TUNEL-positive cells (A) and the expression level of cleaved caspase-3 (B). The blue staining (DAPI) identifies cell nuclei. (C) The expression level of Msi1 was assessed by western blot. 4 µM purmorphamine treatment for 72 h significantly increased Msi1 expression. β-actin was used as an internal control. The results are shown as the mean ± SD from three independent experiments. * P<0.05, ** P<0.01, and *** P<0.001.