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. 2013 Feb 13;8(2):e56496. doi: 10.1371/journal.pone.0056496

Figure 3. The effects of Msi1 knockdown on cell proliferation and apoptosis.

Figure 3

After preculture for 24 h, hUCB-MSCs were transfected with 50 nM Msi1 siRNA for 48 h. The transfection of 50 nM Msi1 siRNA efficiently reduced Msi1 protein levels. The transfection of 50 nM Msi1 siRNA also significantly increased the number of TUNEL-positive cells (A), as well as the expression level of activated caspase-3 (B), compared with the controls. (C) The transfection of 50 nM Msi1 siRNA significantly increased cell proliferation compared to controls. The blue staining (DAPI) identifies cell nuclei. β-actin was used as an internal control. The results are shown as the mean ± SD from three independent experiments. * P<0.05, ** P<0.01, and *** P<0.001.