Figure 5. The effects of purmorphamine and Msi1 knockdown on p21^CIP1,WAF1 and c-Myc expression.

hUCB-MSCs and hDFs were incubated in standard culture medium with or without 4 µM purmorphamine for 3 days. p21^CIP1,WAF1 and c-Myc expressions levels were then assessed by western blotting. Purmorphamine treatment decreased and increased the expression of p21^CIP1,WAF1 (A) and c-Myc (B), respectively. After preculture for 24 h, hUCB-MSCs were transfected with 50 nM Msi1 siRNA for 48 h. The transfection of 50 nM Msi1 siRNA significantly increased and decreased the expression levels of p21^CIP1,WAF1 (C) and c-Myc (D), respectively. β-actin was used as an internal control. The results are shown as the mean ± SD from three independent experiments. * P<0.05, ** P<0.01, and *** P<0.001.