Skip to main content
. 2013 Feb 13;8(2):e56474. doi: 10.1371/journal.pone.0056474

Figure 1. LPS analysis of S. Enteritidis strains overexpressing RcsB (A) or PmrA (B) protein.

Figure 1

Equal amount of LPS was loaded in each lane and analyzed by Tricine/SDS-PAGE on a 14% (w/v) acrylamide gel followed by silver staining. The concentration of LPS was determined by measuring KDO using the purpald assay. A. Lanes 1–4: bacteria grown in N-minimal medium containing 10 mM MgCl2; lanes 5–8: bacteria grown in N-minimal medium containing 10 µM MgCl2 100 µM FeSO4. B. Lanes 1–4: bacteria grown in N-minimal medium containing 10 mM MgCl2; lanes 5–7: bacteria grown in N-minimal medium containing 10 µM MgCl2 100 µM FeSO4. Plasmids pIZ833, prcsB and ppmrA bears the dam, rcsB and pmrA genes respectively.