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. 2013 Feb 13;8(2):e56474. doi: 10.1371/journal.pone.0056474

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© 2013 Sarnacki et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Equal amount of LPS was loaded in each lane and analyzed by Tricine/SDS-PAGE on a 14% (w/v) acrylamide gel followed by silver staining. The concentration of LPS was determined by measuring KDO using the purpald assay. Plasmids prcsB and ppmrA bears the rcsB and pmrA genes respectively.