Table 1. Bacterial strains and plasmids used in this study.

	Relevant characteristic(s)	Reference/Source
Strain
S. enterica serovar Enteritidis
#5694	Wild type	Dr. F. Collins collection
#SS218	Wild type	Poultry isolate
#SS219	Wild type	Poultry isolate
#SS220	Wild type	Poultry isolate
SEΔdam	#5694 Δdam	[39]
SEΔrcsB	#5694 ΔrcsB	This work
SEΔpmrA	#5694 ΔpmrA	This work
SEΔrcsBΔpmrA	#5694 ΔrcsB ΔpmrA	This work
SEΔwzz	#5694 Δwzzst	[39]
SEpmrA3×FLAG	#5694 pmrA::3×FLAG, Kmr	This work
SEpmrA3×FLAG Δdam	#5694 pmrA::3×FLAG Δdam, Kmr	This work
SErcsB3×FLAG	#5694 rcsB::3×FLAG, Kmr	This work
SE218ΔrcsB	#SS218 ΔrcsB	This work
SE218ΔpmrA	#SS218 ΔpmrA	This work
SE219ΔrcsB	#SS219 ΔrcsB	This work
SE219ΔΔpmrA	#SS219 ΔpmrA	This work
SE220ΔrcsB	#SS220 ΔrcsB	This work
SE220ΔpmrA	#SS220 ΔpmrA	This work
SErcsB3×FLAG Δdam	#5694 rcsB::3×FLAG Δdam, Kmr	This work
Escherichia coli K-12
DH5α	F- φ80lacZM15 endA recA hsdR(rK −mK −) supE thi gyrA relA Δ(lacZYA-argF) U169	Laboratory stock
Plasmids
pCP20	FLP +, λcI857+, λpR Repts, Ampr, Cmr	[40]
pIZ833	E. coli dam gene, Ampr	[69]
pKD3	Template plasmid for mutagenesis, Ampr, Cmr	[40]
pKD4	Template plasmid for mutagenesis, Ampr, Kmr	[40]
pKD46	γ, β, and exo from λ phage, araC-ParaB, Ampr	[40]
pSUB11	3×FLAG FRT ahp FRT bla R6KoriV	[41]
pUC18	High copy number cloning vector, Ampr	GenBank/EMBL sequence accession number L09136
prcsB	rcsB; Ampr (pUC18 backbone)	This work
prcsBas	rcsB cloned in antisense orientation to the Plac promoter; Ampr (pUC18 backbone)	This work
ppmrA	pmrA; Ampr (pUC18 backbone)	This work
ppmrAas	pmrA cloned in antisense orientation to the Plac promoter; AmpR (pUC18 backbone)	This work