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. 2013 Feb 13;8(2):e50564. doi: 10.1371/journal.pone.0050564

Figure 2. Electrophoresis of RNA extracted from breast milk samples.

Figure 2

RNA integrity gels were employed in assessing for the integrity of RNA species. smRNA bands are shown, and ribosomal RNA identified with blue arrows. Lanes 1–4: Exosome precipitation with Exoquick system on whole fresh breast milk with a 2 hour precipitation (Lanes 1&2) versus precipitation overnight (Lanes 3 &4). Lanes 5&6: Trizol reagent smRNA preparation from lipid breast milk fraction. Lanes 7–10: mirVANA smRNA preparation from lipid fractions of fresh (Lanes 7 & 8) and previously frozen (Lanes 9&10) breast milk.