Figure 4. Fasudil induces the regulatory T cells and inhibits IL-17 T cells.

Chronic EAE was induced in C57BL/6 mice with MOG_35–55. Fasudil was injected (i.p.) at 40 mg/kg/d every other day on days 3 and 11 p.i. Mice were sacrificed and splenic MNCs were prepared on days 9 (a) and 21 (b) p.i. Cells were stained with T cell marker CD4 and CD25, IL-10 and IL-17, and subsets of T cells were analyzed using flow cytometry. Scatterplot, representative dot plots from one of three experiments with similar results. Histogram, quantitative results are analyzed for 5 mice in each group. Results are expressed as the percentage of double positive cells in four-quadrant diagram or within rectangle. *p<0.05, **p<0.01, ***p<0.001.