Figure 6. Fasudil enhances the ratio of Arg-1/iNOS.

Chronic EAE was induced in C57BL/6 mice with MOG_35–55. Fasudil was injected (i.p.) at 40 mg/kg/d every other day on days 3 and 11 p.i. On day 21 p.i., mice were sacrificed, and protein was extracted from splenic macrophages and spinal cords. a) Representative bands of Western blot for iNOS and Arg-1 from one of two experiments with similar results. Quantitative analysis of iNOS and Arg-1 expression in splenic macrophages and spinal cords are analyzed for 4–5 mice in each group. Results are expressed as fold change relative to GAPDH as the loading control. *p<0.05, **p<0.01, ***p<0.001. b) The double staining of CD68 (green) and iNOS/Arg-1 (red) by immunohistochemistry. Representative patterns were obtained from one of three experiments with similar results.