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. 2013 Feb 13;8(2):e55992. doi: 10.1371/journal.pone.0055992

Figure 6. Effect of HAT co-activators on PSG5 promoter activation mediated by Sp1 and KLF6.

Figure 6

JEG-3 cells were transfected with the UB5luc plasmid and the expression vectors coding for Sp1 (A) or KLF6 (B) alone (lane 1) or together with either wild-type (lanes 2, 4, and 6) or HAT-deficient (lanes 3, 5, and 7) versions of p300 (light grey bars), CBP (dark grey bars) or PCAF (black bars). Data are shown as relative luciferase activity (RLA) to that of JEG-3 cultures co-transfected with the corresponding empty vectors defined as 1 (dashed line). Results are presented as mean ± SD of one representative experiment of two independent assays performed in triplicates, with consistent results. Statistical differences (p≤0.05) were identified using two-sided Student’s t-test. * depicts statistically different values compared to that of the culture co-transfected with the corresponding empty vectors. † represents statistically different values compared to that of the culture co-transfected with the wild type version of the corresponding co-activator. ‡ shows statistically different values compared to that of the culture co-transfected with the Sp1 or KLF6 expression vector alone.