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. 2013 Feb 13;8(2):e56038. doi: 10.1371/journal.pone.0056038

Figure 7. Transcriptional analysis of the mhb and pca genes during LB400 growth on hydroxybenzoates, gentisate and protocatechuate.

Figure 7

LB400 cells were grown on 3-HBA (lane 1), 4-HBA (lane 2), gentisate (lane 3), protocatechuate (lane 4) and glucose (lane 5) as sole carbon source. (A) Transcription of mhbD and mhbR genes; (B) Transcription of pcaG, pcaQ and pcaR genes. (C) Transcription of mhbM and pobA genes. RT-PCR (15, 20 and 25 amplification cycles) assays were performed using RNA extracted from LB400 cells collected at mid exponential growth phase. Transcription of 16S rRNA gene was used as control (not shown).