Table 2. Predicted genes encoding the gentisate and protocatechuate pathways and peripheral reactions from B. xenovorans LB400.
| Gene* | ORF | aa | Related gene products | |||
| Protein | Function | Organism (Reference) | % Id (aa) | |||
| mhbR1 mhbR2 | BxeA2624 BxeA4529 | 166 | MhbR | Transcriptional regulator, MarR family | Polaromonas naphthalenivorans CJ2 [28] | 30 (161) |
| mhbT1 mhbT2 | BxeA2625BxeA4528 | 466 | PcaK | 4-Hydroxybenzoate transporter | Pseudomonas putida PRS2000 [29] | 33 (448) |
| mhbD1 mhbD2 | BxeA2627BxeA4526 | 348 | MhbD | Gentisate-1,2-dioxygenase | Klebsiella pneumoniae M5a1 [30] | 64 (345) |
| mhbH1 mhbH2 | BxeA2628BxeA4525 | 232 | MhbH | Fumarylpyruvate hydrolase | K. pneumoniae M5a1 [30] | 63 (232) |
| mhbI1mhbI2 | BxeA2629BxeA4524 | 216 | MhbI | Maleylpyruvate isomerase | K. pneumoniae M5a1 [30] | 53 (214) |
| mhbM | BxeB2526 | 396 | MhbM | 3-Hydroxybenzoate 6- hydroxylase | K. pneumoniae M5a1 [30] | 38(397) |
| pcaG | BxeB2776 | 195 | PcaG | Protocatechuate 3,4- dioxygenase, alpha subunit | Acinetobacter lwoffii K24 [31] | 90 (132) |
| pcaH | BxeB2775 | 234 | PcaH | Protocatechuate 3,4- dioxygenase, beta subunit | A. lwoffii K24 [31] | 96 (226) |
| pcaQ | BxeB2772 | 310 | PcaQ | Transcriptional regulator, LysR family | Agrobacterium tumefaciens A348 [32] | 48 (311) |
| pcaF1 | BxeB2167 | 400 | PcaF | β-Ketoadipyl-CoA thiolase | P. putida PRS2000 [33] | 71 (400) |
| pcaF2 | BxeA0469 | 400 | PcaF | β-Ketoadipyl- CoA thiolase | P. putida PRS2000 [33] | 69 (400) |
| pcaF3 | BxeA4255 | 402 | PcaF | β-Ketoadipyl-CoA thiolase | P. putida PRS2000 [33] | 42 (400) |
| pcaC | BxeB0647 | 130 | PcaC | 4-Carboxymucolactone decarboxylase | Acinetobacter baylyi ADP1 [34] | 54 (134) |
| pcaD1 | BxeB0646 | 263 | PcaD | β-Ketoadipate enol-lactone hydrolase | A. baylyi ADP1 [35] | 38 (266) |
| pcaD2 | BxeB0595 | 265 | PcaD | β-Ketoadipate enol-lactone hydrolase | A. baylyi ADP1 [35] | 31 (266) |
| pcaB1 | BxeB0645 | 459 | PcaB | 3-Carboxy-cis,cis-muconate cycloisomerase | P. putida PRS2000 [36] | 52 (422) |
| pcaB2 | BxeB1906 | 445 | PcaB | 3-Carboxy-cis,cis-muconate cycloisomerase | P. putida PRS2000 [36] | 34 (422) |
| pcaJ1 | BxeB0644 | 219 | PcaJ | β-Ketoadipate:succinyl-CoA transferase, beta subunit | P. putida PRS2000 [37] | 77 (213) |
| pcaJ2 | BxeC0574 | 265 | PcaJ | β-Ketoadipate:succinyl-CoA transferase, beta subunit | P. putida PRS2000 [37] | 53 (213) |
| pcaJ3 | BxeA1366 | 234 | PcaJ | β-Ketoadipate:succinyl-CoA transferase, beta subunit | P. putida PRS2000 [37] | 44 (213) |
| pcaI1 | BxeB0643 | 233 | PcaI | β-Ketoadipate:succinyl-CoA transferase, alpha subunit | P. putida PRS2000 [37] | 79 (231) |
| pcaI2 | BxeC0572 | 235 | PcaI | β-Ketoadipate:succinyl-CoA transferase, alpha subunit | P. putida PRS2000 [37] | 49 (231) |
| pcaR | BxeB0642 | 309 | PcaR | Transcriptional regulator, LysR family | Burkhoderia phytofirmans PsJN [38] | 95 (309) |
| pobA | BxeA2040 | 394 | PobA | 4-Hydroxybenzoate-3-monooxygenase | P. fluorescens Pf-5 [21] | 51 (394) |
*
Genes for those cases where biochemical or genetic evidence for function is available are underlined.