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. 2013 Feb 13;8(2):e55940. doi: 10.1371/journal.pone.0055940

Figure 4. High throughput screening compound validation.

Figure 4

The HeLa (FXN-EGFP) stable cell line was exposed to various concentrations of compounds identified by high throughput screening. Cultures were incubated for 72 hours. The levels of EGFP expression were measured by flow cytometry. For each compound the lowest concentration that produced the greatest change in FXN-EGFP expression is shown. Assays were performed in triplicate on at least three independent occasions. Error bars represent standard error of the mean. *p<0.05, **p<0.01 in comparison to the untreated control.