Fig. 3.

Quantitative U937 monocyte adhesion assay in tumor necrosis factor-α (TNF-α)-stimulated human umbilical vein endothelial cells (HUVECs). (A) The intensity of fluorescence labeled-adherent U937 monocytes was measured with a fluorometer (ex 485/em 530 nm). (B) U937 cells adherent to HUVECs observed under a fluorescent microscope at × 100 magnification. Data are presented as means ± SEMs (n = 5). ^*P < 0.01 compared with TNF-α-treated group as determined by one-way analysis of variance followed by Dunnett's test.