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. Author manuscript; available in PMC: 2013 Aug 3.
Published in final edited form as: Circ Res. 2012 Jun 20;111(4):426–436. doi: 10.1161/CIRCRESAHA.112.269399

Figure 1. Ex vivo isolation of pure blood vascular and lymphatic endothelial cells from mouse colon tissue by high-speed cell sorting.

Figure 1

(A) Colon single-cell suspensions were immunostained for the pan-endothelial marker CD31, the leukocyte marker CD45 and the lymphatic marker podoplanin. The CD31+CD45− population was further separated into podoplanin+ LECs and podoplanin− BECs. (B) qPCR for the blood vessel marker VEGFR1 and the lymphatic markers podoplanin and LYVE-1 was performed on sorted LECs and BECs. Four animal-matched pairs of LECs (gray bars) and BECs (white bars) showed consistent differential expression levels. Beta-actin was used for normalization of the expression levels. Error bars show standard deviation.