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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Stem Cells. 2013 Feb;31(2):282–292. doi: 10.1002/stem.1284

Figure 4. Tcea3 is a component of RNA polymerase II transcription complex and regulates expression of Lefty1 in mESCs.

Figure 4

(A) Agarose gel analysis of Tcea3 binding proteins from mESCs. mESC total cell extracts were used as “prey” and GST fused Tcea3 were used as “bait” for the GST pull down assay. (B) List of representative proteins identified as protein binding partners of Tcea3 by the mass spectrometric analysis of peptides extracted from four agarose bands of (A). (C) Scatter plots of cDNA microarray analysis of Tcea3 OE mESCs revealed that Lefty1 expression is most robustly upregulated (R2 = correlation coefficients).