Fig. 3.

Characterization of mitochondria and metabolic features. Levels of A) reactive oxygen species (ROS), B) oxidized/reduced glutathione (GSSG/GSH), C) ATP, D) lipid content and E) lysosome-related organelles (LROs) in 1-day-old adult WT animals fed bacteria expressing either empty-vector (con) or life-lengthening frh-1 dsRNA (frh-1). RFU, Relative Fluorescence Unit. RLU, Relative Luminescence Unit. F–I) Transcript expression of key metabolic enzymes in animals treated as in A. Bars and errors indicate mean fold changes and the 95% confidence interval of the mean, respectively, of cumulative data from three independent experiments. *p < 0.05 of each single gene vs control, t-test.