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. 2013 Feb 1;4(2):152–164. doi: 10.7150/jca.5702

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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RT-PCR analysis of the mRNA levels of human HtrA1, HtrA2 and HrA3 [long (HtrA3-L) and short (HtrA3-S)] in various cancer cell lines. (A) Representative semi-quantitative PCR results from testes, breast, prostate, and colon control and cancer cell lines. (B) Representative semi-quantitative PCR results from ovarian and endometrial control and cancer cell lines. 18S amplification was used as loading control. Pre-menopausal and post-menopausal ovary - normal controls for ovary; Mid-Proliferative and Mid-Secretory - normal controls for mid proliferative and secretory phase endometrium respectively. Cancer cell lines examined, testes - Ntera-2D; breast - MCK-7, MDA-231; prostate - PC3, DU145, and LNCap; colon - SW480 and WIDR; ovary - KGN and COV434; endometrium - ECC1, HEC1-A, HEC1-B, Ishikawa, RL95-2 and AN3CA.