Table 2.
The primer sequences and reaction conditions for RT-PCR.
| Gene | Primer Sequences (5' - 3') F- Forward, R- Reverse |
Annealing temperature (0C) and cycles (n) - Block PCR | PCR Product size (bp) |
|---|---|---|---|
| HTRA1 | F - AAA GCC ATC ACC AAG AAG AAG TAT R - TCC TCA TCC GTC ATC CAC |
58 (30 cycles) | 384 |
| HTRA2 | F - GCC GTG GTC TAT ATC GAG ATC CT R - TGC CGG ATG TGA TCG TGT |
58 (30 cycles) | 343 |
| HTRA3-L (Long) | F - ATG CGG ACG ATC ACA CCA AG R - CGC TGC CCT CCG TTG TCT G |
58 (30 cycles) | 337 |
| HTRA3-S (short) | F - GAG GGC TGG TCA CAT GAA GA R - GCT CCG CTA ATT TCC AGT |
53 (35 cycles) | 320 |
| 18S | F - CGG CTA CCA CAT CCA AGG AA R - GCT GGA ATT ACC GCG GCT |
64 (26 cycles) | 187 |