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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Neurobiol Dis. 2012 Apr 6;47(2):145–154. doi: 10.1016/j.nbd.2012.03.032

Figure 6.

Figure 6

Aberrant mossy fibers sprouting decreased in the dentate gyrus of epileptic EAAT2 mice. Timm staining was performed to detect mossy fiber spouting with mice harvested at 8 weeks after SE. Sections were scored by 2 investigators independently on a scale of 0~5 (Fig. S1). (A-C) Representative images of Timm-stained dentate gyrus. scale bar, 50 μm. (D) EAAT2 mice exhibited significantly lower Timm scores compared to wild-type littermates (n=16, *p<0.05, Dunn’s method). (E) A moderate but not significant positive correlation (correlation coefficient=0.45, p=0.052, Spearman rank order correlation test) was detected between the severity of aberrant mossy fiber sprouting and chronic seizure frequency. (F) A strong positive correlation was detected between the hilar cell loss and the Timm score (correlation coefficient=0.59, p<0.001, Spearman rank order correlation test).