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Thirty minutes after i.c.v injection of T1AM (0.04, 0.13 μg·kg−1) or vehicle, mice (n = 3 per group) were killed and brain regions separated as described in the Methods section. Immunodetection for pERK was carried out on protein lysates from each region separated on SDS-PAGE gels. Results are the mean ± SEM of the densitometry of three different gels. (A) Results of a typical experiment. (B) Cumulative results. *P < 0.05 versus vehicle.
