Figure 1.
Effect of saline or PK2 on c-fos-like immunoreactivity in the brain of mice. (A, B) Representative light-field microphotographs showing the effect of i.p. saline (A) or 540 nmol kg−1 PK2 (B) administration on c-fos-like immunoreactivity in the brain of mice. The arrows indicate cfos-like immunoreactivity in the DMV nucleus of the brainstem in PK2-treated animals. AP = area postrema, CC = central canal. A and B: 200× magnification. Scale bars are equivalent to 100 μm, n = 6 per group. (C) Median and interquartile ranges of c-fos-like immunoreactivity in DMV, PVN, SON, AHA, suprachiasmatic nucleus (SCN), ARC, ventromedial nucleus (VMH), dorsomedial nucleus (DMH) and lateral hypothalamic area (LHA). The saline and 540 nmol kg−1 PK2 groups are denoted by the left and right bars in each pair respectively.