Figure 5.

Radioactive anti-TENB2 was stable in plasma over the course of the study, and its catabolites were excreted in urine. No plasma protein complex formation was evident by HPLC analysis at 24 (red), 48 (blue) and 72 (green) h post-injection. Consistent presence of the radioimmunoconjugate [retention time (RT) ∼17 min, see Supporting Information Fig. S4] was observed in plasma of mice receiving [¹¹¹In]-anti-TENB2-MMAE (ADC) at tracer only (A) or 10 mg·kg⁻¹ (B) dose levels. In contrast, no intact protein and only radiolabelled catabolites (RT ∼24 min) were observed in urine from mice receiving tracer only (C) or 10 mg·kg⁻¹ (D) dose. Note the difference in y-axis scale, particularly between (C) and (D).